At the summer workshop of the Korean Endocrine Society held in 2016, some examples of protein experiments were discussed in the session entitled “All about working with proteins.” In contrast to what the title suggested, it was unrealistic to comprehensively discuss all protein analytical methods. Therefore, the goal was to outline protein experimental techniques that are useful in research or in bench work. In conversations with clinicians, however, I have always felt that researchers who do not engage in bench science have different demands than those who do. Protein research tools that are useful in bench science may not be very useful or effective in the diagnostic field. In this paper, I provide a general summary of the protein analytical methods that are used in basic scientific research, and describe how they can be applied in the diagnostic field.

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• Functional Properties of Moringa Oleifera Protein Isolates as Influenced by Different Isolation Techniques, pH, and Ionic Strength
  Kirsty A. Illingworth, Yee Ying Lee, Lee Fong Siow
  Food and Bioprocess Technology.2024;[Epub]     CrossRef
• Evolving Paradigms of Recombinant Protein Production in Pharmaceutical Industry: A Rigorous Review
  Achuth Jayakrishnan, Wan Rosalina Wan Rosli, Ahmad Rashidi Mohd Tahir, Fashli Syafiq Abd Razak, Phei Er Kee, Hui Suan Ng, Yik-Ling Chew, Siew-Keah Lee, Mahenthiran Ramasamy, Ching Siang Tan, Kai Bin Liew
  Sci.2024; 6(1): 9.     CrossRef
• Proteomics techniques in protein biomarker discovery
  Mahsa Babaei, Soheila Kashanian, Huang‐Teck Lee, Frances Harding
  Quantitative Biology.2024; 12(1): 53.     CrossRef
• Functional Studies of Cytogenotoxic Potential of Laticifer Proteins of Calotropis procera against Viral Disease
  Uzma Saher, Muhammad Ovais Omer, Aqeel Javeed, Aftab Ahmad Anjum, Kanwal Rehman, Tanzeela Awan, Gulbeena Saleem, Ameena Mobeen
  ACS Omega.2023; 8(7): 7119.     CrossRef
• Advances and key considerations of liquid chromatography–mass spectrometry for porcine authentication in halal analysis
  Kifayati Rosiyanti Dewi, Maya Ismayati, Nissa Nurfajrin Solihat, Nancy Dewi Yuliana, Feri Kusnandar, Handy Riantana, Heryani Heryani, Abdul Halim, Thamina Acter, Nizam Uddin, Sunghwan Kim
  Journal of Analytical Science and Technology.2023;[Epub]     CrossRef
• A Path to Protein Quantitation: PBMCs to Western Blot
  Sufaya Jameel, Parveen Jahan, Subbiah Ramasamy
  International Journal of Methodology.2023; 2(1): 12.     CrossRef
• Obtención y caracterización de un hidrolizado de proteína de Vigna radiata L. (frijol mungo) con potencial Bioestimulante
  F.C. Arámbula-Castillo, M. Zegbe-Rodríguez, C. Rivas-Morales, D.G. García-Hernández , E.D. Cabello-Ruiz
  Investigación y Desarrollo en Ciencia y Tecnología de Alimentos.2023; 8(1): 170.     CrossRef
• Optimization and evaluation of tear protein elution from Schirmer’s strips in dry eye disease
  Madhuri Amulya Koduri, Deeksha Prasad, Tejaswini Pingali, Vijay Kumar Singh, Swapna S Shanbhag, Sayan Basu, Vivek Singh
  Indian Journal of Ophthalmology.2023; 71(4): 1413.     CrossRef
• Actinobacillus pleuropneumoniae, surface proteins and virulence: a review
  María M. Soto Perezchica, Alma L. Guerrero Barrera, Francisco J. Avelar Gonzalez, Teodulo Quezada Tristan, Osvaldo Macias Marin
  Frontiers in Veterinary Science.2023;[Epub]     CrossRef
• Emerging on‐chip electrokinetic based technologies for purification of circulating cancer biomarkers towards liquid biopsy: A review
  Leilei Shi, Leyla Esfandiari
  ELECTROPHORESIS.2022; 43(1-2): 288.     CrossRef
• Mag-spinner: a next-generation Facile, Affordable, Simple, and porTable (FAST) magnetic separation system
  Sanghoon Lee, Miseon Jeong, Soojin Lee, Sang Hun Lee, Jin-sil Choi
  Nanoscale Advances.2022; 4(3): 792.     CrossRef
• Leveraging multiomics approaches for producing lignocellulose degrading enzymes
  Kavya Dashora, Meghana Gattupalli, Zoya Javed, Gyan Datta Tripathi, Ruchi Sharma, Mansi Mishra, Atul Bhargava, Shilpi Srivastava
  Cellular and Molecular Life Sciences.2022;[Epub]     CrossRef
• Mass spectrometric profiling of tryptic digests of trifluoroethanol extracts from core needle biopsies of breast cancer tissues is a viable sample screening tool for biomarker discovery
  Prarthana Gopinath, Gopal Gopisetty, Sridevi Veluswami, Shirley Sundersingh, Rajkumar Thangarajan
  Journal of Proteins and Proteomics.2022; 13(2): 79.     CrossRef
• The effect of isolation techniques on the physicochemical properties of Moringa oleifera protein isolates
  Kirsty A. Illingworth, Yee Ying Lee, Lee Fong Siow
  Food Chemistry Advances.2022; 1: 100029.     CrossRef
• Physical, Chemical and Biochemical Modification Approaches of Potato (Peel) Constituents for Bio-Based Food Packaging Concepts: A Review
  Katharina Miller, Corina L. Reichert, Markus Schmid, Myriam Loeffler
  Foods.2022; 11(18): 2927.     CrossRef
• Identification of transient receptor potential melastatin 3 proteotypic peptides employing an efficient membrane protein extraction method for natural killer cells
  Chandi T. Magawa, Natalie Eaton-Fitch, Cassandra Balinas, Etianne Martini Sasso, Kiran Thapaliya, Leighton Barnden, Rebekah Maksoud, Breanna Weigel, Penny A. Rudd, Lara J. Herrero, Sonya Marshall-Gradisnik
  Frontiers in Physiology.2022;[Epub]     CrossRef
• Comparative Analysis of Universal Protein Extraction Methodologies for Screening of Lipase Activity from Agricultural Products
  Jisu Ha, Jun-Young Park, Yoonseok Choi, Pahn-Shick Chang, Kyung-Min Park
  Catalysts.2021; 11(7): 816.     CrossRef
• Solid-Phase Peptide Capture and Release for Bulk and Single-Molecule Proteomics
  Cecil J. Howard, Brendan M. Floyd, Angela M. Bardo, Jagannath Swaminathan, Edward M. Marcotte, Eric V. Anslyn
  ACS Chemical Biology.2020; 15(6): 1401.     CrossRef
• A flexible, robust microbead-based assay for quantification and normalization of target protein concentrations
  Eric J. Snider, Alexandra R. Crowley, Julia Raykin, R. Kijoon Kim, Fiona Splaine, C. Ross Ethier
  Analytical Biochemistry.2020; 590: 113510.     CrossRef
• Applications of Converged Various Forces for Detection of Biomolecules and Novelty of Dielectrophoretic Force in the Applications
  Seungjun Lee, Seong Min Roh, Eunji Lee, Yejin Park, Byung Chul Lee, Youngeun Kwon, Hye Jin Kim, Jinsik Kim
  Sensors.2020; 20(11): 3242.     CrossRef